How is a slant culture typically prepared?

How is a slant culture typically prepared?

• A. By freezing bacteria in liquid nitrogen
• B. By inoculating a tube and letting it solidify at an angle
• C. By mixing glycerol with liquid cultures
• D. By placing bacteria in a petri dish

Answer: (B)

A slant culture is prepared by inoculating a tube of agar and allowing it to solidify at an angle. This method provides a solid surface area for bacterial growth while minimizing the volume of the culture medium. The tilted surface increases the surface area and allows for better aeration and access for the microorganisms, making it ideal for storing or culturing bacteria. When the agar is allowed to solidify at an angle, it creates a slanted surface, which can accommodate the growth of bacterial colonies. This setup is commonly used in microbiology labs to maintain cultures for longer durations and to allow for easier access when taking samples. The process of preparing slant cultures serves important functions in laboratory settings, including easy transportation, efficient use of space, and an optimal environment for microbial growth.

A slant culture is prepared by inoculating a tube of agar and allowing it to solidify at an angle. This method provides a solid surface area for bacterial growth while minimizing the volume of the culture medium. The tilted surface increases the surface area and allows for better aeration and access for the microorganisms, making it ideal for storing or culturing bacteria.

When the agar is allowed to solidify at an angle, it creates a slanted surface, which can accommodate the growth of bacterial colonies. This setup is commonly used in microbiology labs to maintain cultures for longer durations and to allow for easier access when taking samples.

The process of preparing slant cultures serves important functions in laboratory settings, including easy transportation, efficient use of space, and an optimal environment for microbial growth.