Why is it important to use a fixative in staining procedures?

Why is it important to use a fixative in staining procedures?

• A. To cool the sample
• B. To adhere the stain to the organism
• C. To increase visibility of the sample
• D. To enhance the pH of the solution

Answer: (B)

Using a fixative in staining procedures is crucial because it helps to preserve the structure of the specimen, making it possible for the stain to adhere properly to the organism. Fixatives work by cross-linking proteins and stabilizing cell membranes, which prevents the degradation of cellular components. This stabilization allows for clear and consistent staining, as the stain can bind effectively to the cellular structures without the risk of them disintegrating during the staining process. This adherence is essential for accurate visualization under a microscope, as it ensures that the target structures are highlighted properly and remain intact during the preparation and observation stages.

Using a fixative in staining procedures is crucial because it helps to preserve the structure of the specimen, making it possible for the stain to adhere properly to the organism. Fixatives work by cross-linking proteins and stabilizing cell membranes, which prevents the degradation of cellular components. This stabilization allows for clear and consistent staining, as the stain can bind effectively to the cellular structures without the risk of them disintegrating during the staining process.

This adherence is essential for accurate visualization under a microscope, as it ensures that the target structures are highlighted properly and remain intact during the preparation and observation stages.