Why is tap polymerase significant in PCR?

Why is tap polymerase significant in PCR?

• A. It can withstand high temperatures during the amplification process
• B. It is the first enzyme used in DNA synthesis
• C. It only functions at low temperatures
• D. It produces RNA from DNA

Answer: (A)

Tap polymerase is significant in PCR (Polymerase Chain Reaction) primarily because it can withstand high temperatures during the amplification process. PCR involves repeated cycles of heating and cooling to denature the DNA and allow for annealing of primers, and it is crucial that the polymerase enzyme used in the reaction remains functional at elevated temperatures, particularly around 95°C. Tap polymerase, derived from the Thermus aquaticus bacterium, is a thermostable enzyme that maintains its activity even under these extreme conditions, making it ideal for the repetitive heating required in PCR. This stability allows for efficient DNA synthesis in each cycle of the PCR, ensuring that the amplification of the target DNA sequence is effective and reproducible.

Tap polymerase is significant in PCR (Polymerase Chain Reaction) primarily because it can withstand high temperatures during the amplification process. PCR involves repeated cycles of heating and cooling to denature the DNA and allow for annealing of primers, and it is crucial that the polymerase enzyme used in the reaction remains functional at elevated temperatures, particularly around 95°C. Tap polymerase, derived from the Thermus aquaticus bacterium, is a thermostable enzyme that maintains its activity even under these extreme conditions, making it ideal for the repetitive heating required in PCR. This stability allows for efficient DNA synthesis in each cycle of the PCR, ensuring that the amplification of the target DNA sequence is effective and reproducible.